Loop-mediated Isothermal Amplification and nested PCR of the Internal Transcribed Spacer (ITS) for Histoplasma capsulatum detection

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Development of a loop-mediated isothermal amplification method for detection of Histoplasma capsulatum DNA in clinical samples.

Improved methods for the detection of Histoplasma capsulatum are needed in regions with limited resources in which the organism is endemic, where delayed diagnosis of progressive disseminated histoplasmosis (PDH) results in high mortality rates. We have investigated the use of a loop-mediated isothermal amplification (LAMP) assay to facilitate rapid inexpensive molecular diagnosis of this disea...

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ژورنال

عنوان ژورنال: PLOS Neglected Tropical Diseases

سال: 2019

ISSN: 1935-2735

DOI: 10.1371/journal.pntd.0007692